This guide explains how to use the search features of the InsectDB.
Performing a Search
Enter a Query: You can either paste one or more FASTA-formatted sequences into the text box, or upload a FASTA file.
Select Sequence Types: Specify whether your query and the database you want to search against are 'Protein' or 'Nucleotide'. The tool will automatically select the correct BLAST program (blastp, blastn, tblastn, blastx).
Filter Results: You can set optional filters for E-value, Percent Identity, and Score to narrow down your results.
Interpreting the Results
Query Coverage: This bar visually represents which portion of your query sequence matches the hit.
Alignment: Click the 'Show' button to view the detailed pairwise sequence alignment for any hit.
Domains: For protein hits, click the 'Find' link to perform a search for conserved protein domains using the NCBI CD-Search tool. This will open in a new tab.